Entropy Beacon: A Hairpin-Free DNA Amplification Strategy for Efficient Detection of Nucleic Acids

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• 作者：Yifan Lv ; Liang Cui ; Ruizi Peng ; Zilong Zhao ; Liping Qiu ; Huapei Chen ; Cheng Jin ; Xiao-Bing Zhang ; Weihong Tan
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：December 1, 2015
• 年：2015
• 卷：87
• 期：23
• 页码：11714-11720
• 全文大小：388K
• ISSN：1520-6882

文摘

Here, we propose an efficient strategy for enzyme- and hairpin-free nucleic acid detection called an entropy beacon (abbreviated as Ebeacon). Different from previously reported DNA hybridization/displacement-based strategies, Ebeacon is driven forward by increases in the entropy of the system, instead of free energy released from new base-pair formation. Ebeacon shows high sensitivity, with a detection limit of 5 pM target DNA in buffer and 50 pM in cellular homogenate. Ebeacon also benefits from the hairpin-free amplification strategy and zero-background, excellent thermostability from 20 掳C to 50 掳C, as well as good resistance to complex environments. In particular, based on the huge difference between the breathing rate of a single base pair and two adjacent base pairs, Ebeacon also shows high selectivity toward base mutations, such as substitution, insertion, and deletion and, therefore, is an efficient nucleic acid detection method, comparable to most reported enzyme-free strategies.