Effects of Tris(1,3-dichloro-2-propyl) Phosphate on Growth, Reproduction, and Gene Transcription of Daphnia magna at Environmentally Relevant Concentrations

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• 作者：Han Li ; Guanyong Su ; Ming Zou ; Liqin Yu ; Robert J. Letcher ; Hongxia Yu ; John P. Giesy ; Bingsheng Zhou ; Chunsheng Liu
• 刊名：Environmental Science & Technology
• 出版年：2015
• 出版时间：November 3, 2015
• 年：2015
• 卷：49
• 期：21
• 页码：12975-12983
• 全文大小：502K
• ISSN：1520-5851

文摘

The synthetic flame retardant tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been frequently detected in natural waters, and its maximum concentration ever reported is 377 ng/L. However, information on the adverse effects of environmentally relevant concentrations of TDCIPP on aquatic organisms are totally unknown. In this study, <12-h old water fleas, D. magna, were exposed to concentrations of 0, 65 卤 7.1, 550 卤 33, or 6500 卤 1400 ng/L TDCIPP, and dose- and time-dependent effects on reproduction and development were evaluated. Sequences of genes of D. magna were obtained from the National Center for Biotechnology Information and were used to develop PCR arrays for D. magna. Arrays were then used to study transcriptional responses of D. magna to TDCIPP. Exposure to environmentally relevant concentrations of TDCIPP significantly decreased fecundity as well as length of F₀ and F₁ generations. Transcriptional responses showed that, of the 155 genes tested, expressions of 57 genes were significantly changed, and some changes occurred following exposure to environmentally relevant concentrations (i.e., 65 卤 7.1 and 550 卤 23 ng/L). Furthermore, pathways related to protein synthesis and metabolism and endocytosis were considered to be significantly affected in a dose- and time-dependent manner and might be responsible for TDCIPP-induced reproductive and developmental toxicities.