Identification of Target Proteins of Mangiferin in Mice with Acute Lung Injury Using Functionalized Magnetic Microspheres Based on Click Chemistry

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• 作者：Jiajia Wang ; Yan Nie ; Yunjuan Li ; Yuanyuan Hou ; Wei Zhao ; Jiagang Deng ; Peng George Wang ; Gang Bai
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：November 18, 2015
• 年：2015
• 卷：63
• 期：45
• 页码：10013-10021
• 全文大小：565K
• ISSN：1520-5118

文摘

Prevention of the occurrence and development of inflammation is a vital therapeutic strategy for treating acute lung injury (ALI). Increasing evidence has shown that a wealth of ingredients from natural foods and plants have potential anti-inflammatory activity. In the present study, mangiferin, a natural C-glucosyl xanthone that is primarily obtained from the peels and kernels of mango fruits and the bark of the Mangifera indica L. tree, alleviated the inflammatory responses in lipopolysaccharide (LPS)-induced ALI mice. Mangiferin-modified magnetic microspheres (MMs) were developed on the basis of click chemistry to capture the target proteins of mangiferin. Mass spectrometry and molecular docking identified 70 kDa heat-shock protein 5 (Hspa5) and tyrosine 3-monooxygenase (Ywhae) as mangiferin-binding proteins. Furthermore, an enzyme-linked immunosorbent assay (ELISA) indicated that mangiferin exerted its anti-inflammatory effect by binding Hspa5 and Ywhae to suppress downstream mitogen-activated protein kinase (MAPK) signaling pathways. Thoroughly revealing the mechanism and function of mangiferin will contribute to the development and utilization of agricultural resources from M. indica L.