Lotus Leaf Alkaloid Extract Displays Sedative鈥揌ypnotic and Anxiolytic Effects through GABAA Receptor

详细信息    查看全文

• 作者：Ming-Zhu Yan ; Qi Chang ; Yu Zhong ; Bing-Xin Xiao ; Li Feng ; Fang-Rui Cao ; Rei-Le Pan ; Ze-Sheng Zhang ; Yong-Hong Liao ; Xin-Min Liu
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：October 28, 2015
• 年：2015
• 卷：63
• 期：42
• 页码：9277-9285
• 全文大小：466K
• ISSN：1520-5118

文摘

Lotus leaves have been used traditionally as both food and herbal medicine in Asia. Open-field, sodium pentobarbital-induced sleeping and light/dark box tests were used to evaluate sedative鈥揾ypnotic and anxiolytic effects of the total alkaloids (TA) extracted from the herb, and the neurotransmitter levels in the brain were determined by ultrafast liquid chromatography鈥搕andem mass spectrometry. The effects of picrotoxin, flumazenil, and bicuculline on the hypnotic activity of TA, as well as the influence of TA on Cl^{鈥?/sup> influx in cerebellar granule cells, were also investigated. TA showed a sedative鈥揾ypnotic effect by increasing the brain level of 纬-aminobutyric acid (GABA), and the hypnotic effect could be blocked by picrotoxin and bicuculline, but could not be antagonized by flumazenil. Additionally, TA could increase Cl鈥?/sup> influx in cerebellar granule cells. TA at 20 mg/kg induced anxiolytic-like effects and significantly increased the concentrations of serotonin (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), and dopamine (DA). These data demonstrated that TA exerts sedative鈥揾ypnotic and anxiolytic effects via binding to the GABA_A receptor and activating the monoaminergic system.}