Phosphorylated Peptides from Antarctic Krill (Euphausia superba) Prevent Estrogen Deficiency Induced Osteoporosis by Inhibiting Bone Resorption in Ovariectomized Rats

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• 作者：Guanghua Xia ; Yanlei Zhao ; Zhe Yu ; Yingying Tian ; Yiming Wang ; Shanshan Wang ; Jingfeng Wang ; Changhu Xue
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：November 4, 2015
• 年：2015
• 卷：63
• 期：43
• 页码：9550-9557
• 全文大小：580K
• ISSN：1520-5118

文摘

In the current study, we investigated the improvement of phosphorylated peptides from Antarctic krill Euphausia superba (PP-AKP) on osteoporosis in ovariectomized rats. PP-AKP was supplemented to ovariectomized Sprague鈥揇awley rats for 90 days. The results showed that PP-AKP treatment remarkably prevented the reduction of bone mass and improved cancellous bone structure and biochemical properties. PP-AKP also significantly decreased serum contents of tartrate-resistant acid phosphatase (TRACP), cathepsin K (Cath-k), matrix metalloproteinases-9 (MMP-9), deoxypyridinoline (DPD), C-terminal telopeptide of collagen I (CTX-1), Ca, and P. Mechanism investigation revealed that PP-AKP significantly increased the osteoprotegerin (OPG)/receptor activator of nuclear factor 魏B ligand (RANKL) ratio in mRNA expression, protein expression, and serum content. Further research suggested that NF-魏B signaling pathways were inhibited by suppressing the mRNA and protein expressions of nuclear factor of activated T-cells (NFATc1) and tumor necrosis factor receptor-associated factor 6 (TRAF6), diminishing the mRNA expression and phosphorylation of nuclear factor 魏B p65 (NF-魏B p65), three key transcription factors in NF-魏B pathways. These results suggest that PP-AKP can improve osteoporosis by inhibiting bone resorption via suppressing the activation of osteoclastogenesis related NF-魏B pathways.