An Interface Coassembly in Biliquid Phase: Toward Core鈥揝hell Magnetic Mesoporous Silica Microspheres with Tunable Pore Size

详细信息    查看全文

• 作者：Qin Yue ; Jialuo Li ; Wei Luo ; Yu Zhang ; Ahmed A. Elzatahry ; Xiqing Wang ; Chun Wang ; Wei Li ; Xiaowei Cheng ; Abdulaziz Alghamdi ; Aboubakr M. Abdullah ; Yonghui Deng ; Dongyuan Zhao
• 刊名：Journal of the American Chemical Society
• 出版年：2015
• 出版时间：October 21, 2015
• 年：2015
• 卷：137
• 期：41
• 页码：13282-13289
• 全文大小：525K
• ISSN：1520-5126

文摘

Core鈥搒hell magnetic mesoporous silica microspheres (Magn-MSMs) with tunable large mesopores in the shell are highly desired in biocatalysis, magnetic bioseparation, and enrichment. In this study, a shearing assisted interface coassembly in n-hexane/water biliquid systems is developed to synthesize uniform Magn-MSMs with magnetic core and mesoporous silica shell for an efficient size-selective biocatalysis. The synthesis features the rational control over the electrostatic interaction among cationic surfactant molecules, silicate oligomers, and Fe₃O₄@RF microspheres (RF: resorcinol formaldehyde) in the presence of shearing-regulated solubilization of n-hexane in surfactant micelles. Through this multicomponent interface coassembly, surfactant-silica mesostructured composite has been uniformly deposited on the Fe₃O₄@RF microspheres, and core鈥搒hell Magn-MSMs are obtained after removing the surfactant and n-hexane. The obtained Magn-MSMs possess excellent water dispersibility, uniform diameter (600 nm), large and tunable perpendicular mesopores (5.0鈥?.0 nm), high surface area (498鈥?23 m²/g), large pore volume (0.91鈥?.98 cm³/g), and high magnetization (34.5鈥?7.1 emu/g). By utilization of their large and open mesopores, Magn-MSMs with a pore size of about 9.0 nm have been demonstrated to be able to immobilize a large bioenzyme (trypsin with size of 4.0 nm) with a high loading capacity of 鈭?7 渭g/mg via chemically binding. Magn-MSMs with immobilized trypsin exhibit an excellent convenient and size selective enzymolysis of low molecular proteins in the mixture of proteins of different sizes and a good recycling performance by using the magnetic separability of the microspheres.