Integrated Proteomic and Genomic Analysis of Gastric Cancer Patient Tissues

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• 作者：Julia Fangfei Yan ; Hoguen Kim ; Seul-Ki Jeong ; Hyoung-Joo Lee ; Manveen K. Sethi ; Ling Y. Lee ; Ronald C. Beavis ; Hogune Im ; Michael P. Snyder ; Matan Hofree ; Trey Ideker ; Shiaw-lin Wu ; Young-Ki Paik ; Susan Fanayan ; William S. Hancock
• 刊名：Journal of Proteome Research
• 出版年：2015
• 出版时间：December 4, 2015
• 年：2015
• 卷：14
• 期：12
• 页码：4995-5006
• 全文大小：749K
• ISSN：1535-3907

文摘

V-erb-b2 erythroblastic leukemia viral oncogene homologue 2, known as ERBB2, is an important oncogene in the development of certain cancers. It can form a heterodimer with other epidermal growth factor receptor family members and activate kinase-mediated downstream signaling pathways. ERBB2 gene is located on chromosome 17 and is amplified in a subset of cancers, such as breast, gastric, and colon cancer. Of particular interest to the Chromosome-Centric Human Proteome Project (C-HPP) initiative is the amplification mechanism that typically results in overexpression of a set of genes adjacent to ERBB2, which provides evidence of a linkage between gene location and expression. In this report we studied patient samples from ERBB2-positive together with adjacent control nontumor tissues. In addition, non-ERBB2-expressing patient samples were selected as comparison to study the effect of expression of this oncogene. We detected 196 proteins in ERBB2-positive patient tumor samples that had minimal overlap (29 proteins) with the non-ERBB2 tumor samples. Interaction and pathway analysis identified extracellular signal regulated kinase (ERK) cascade and actin polymerization and actinmyosin assembly contraction as pathways of importance in ERBB2+ and ERBB2鈥?gastric cancer samples, respectively. The raw data files are deposited at ProteomeXchange (identifier: PXD002674) as well as GPMDB.