Colloidal Gold鈥揅ollagen Protein Core鈥揝hell Nanoconjugate: One-Step Biomimetic Synthesis, Layer-by-Layer Assembled Film, and Controlled Cell Growth

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• 作者：Ruirui Xing ; Tifeng Jiao ; Linyin Yan ; Guanghui Ma ; Lei Liu ; Luru Dai ; Junbai Li ; Helmuth M枚hwald ; Xuehai Yan
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：November 11, 2015
• 年：2015
• 卷：7
• 期：44
• 页码：24733-24740
• 全文大小：492K
• ISSN：1944-8252

文摘

The biogenic synthesis of biomolecule鈥揼old nanoconjugates is of key importance for a broad range of biomedical applications. In this work, a one-step, green, and condition-gentle strategy is presented to synthesize stable colloidal gold鈥揷ollagen core鈥搒hell nanoconjugates in an aqueous solution at room temperature, without use of any reducing agents and stabilizing agents. It is discovered that electrostatic binding between gold ions and collagen proteins and concomitant in situ reduction by hydroxyproline residues are critically responsible for the formation of the core鈥搒hell nanoconjugates. The film formed by layer-by-layer assembly of such colloidal gold鈥揷ollagen nanoconjugates can notably improve the mechanical properties and promote cell adhesion, growth, and differentiation. Thus, the colloidal gold鈥揷ollagen nanoconjugates synthesized by such a straightforward and clean manner, analogous to a biomineralization pathway, provide new alternatives for developing biologically based hybrid biomaterials toward a range of therapeutic and diagnostic applications.