Self-Assembled DNA Immunonanoflowers as Multivalent CpG Nanoagents

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• 作者：Liqin Zhang ; Guizhi Zhu ; Lei Mei ; Cuichen Wu ; Liping Qiu ; Cheng Cui ; Yuan Liu ; I-Ting Teng ; Weihong Tan
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：November 4, 2015
• 年：2015
• 卷：7
• 期：43
• 页码：24069-24074
• 全文大小：441K
• ISSN：1944-8252

文摘

Synthetic unmethylated cytosine鈥揼uanine (CpG) oligodeoxynucleotides are immunostimulatory motifs that have shown promise as vaccines or adjuvants for diseases such as cancers and infectious diseases. In the present work, novel immuno-nanoflowers (NFs), self-assembled from long DNA integrated with tandem CpG through rolling circle replication, were developed for efficient CpG delivery and protection from nuclease degradation. In a model of macrophage-like cells, the CpG NFs proved to be potent immunostimulators by triggering the proliferation of these immune cells, which, in turn, secreted immunostimulatory cytokines, including tumor necrosis factor 伪, interleukin-6, and interleukin-10. These results demonstrate the ability of CpG NFs to induce cancer cell apoptosis and necrosis.