Targeting Ability of Affibody-Functionalized Particles Is Enhanced by Albumin but Inhibited by Serum Coronas

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• 作者：Qiong Dai ; Yan Yan ; Junling Guo ; Mattias Bj枚rnmalm ; Jiwei Cui ; Huanli Sun ; Frank Caruso
• 刊名：ACS Macro Letters
• 出版年：2015
• 出版时间：November 17, 2015
• 年：2015
• 卷：4
• 期：11
• 页码：1259-1263
• 全文大小：380K
• ISSN：2161-1653

文摘

Protein coronas formed on engineered particles can alter their targeting ability as they enter biological environments. Here, we engineer polymer-coated silica particles and investigate the influence of protein coronas derived from various sources. The particles were functionalized with a small antibody-mimetic ligand (affibody), and their targeting ability to cancer cells in the presence of protein coronas was determined. Protein coronas derived from human serum showed a dramatic inhibition of specific particle-cell association (from 鈭?0 to 鈭?%), whereas the most abundant protein in human serum鈥攈uman serum albumin鈥攅nhanced the specific association of functionalized particles to SK-OV-3 human ovary cancer cells (to 鈭?0%). This study shows how protein coronas can both facilitate and impede targeting and provides key insights into the importance of challenging engineered particles with multicomponent biologically relevant environments.