Fluorescent pH Sensors for Broad-Range pH Measurement Based on a Single Fluorophore

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• 作者：Jing Qi ; Daying Liu ; Xiaoyan Liu ; Shiquan Guan ; Fengli Shi ; Hexi Chang ; Huarui He ; Guangming Yang
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：June 16, 2015
• 年：2015
• 卷：87
• 期：12
• 页码：5897-5904
• 全文大小：483K
• ISSN：1520-6882

文摘

We constructed a series of novel optical sensors for determination of broad-range pH based on a single fluorophore and multi-ionophores with different pK_a values. These optical sensors use photoinduced electron transfer (PET) as the signal transduction and follow the design concept of 鈥渇luorophore-spacer-receptor (ionophore)鈥?which employs 4-amino-1,8-naphthalimide as the single fluorophore, ethyl moiety as the spacer, and a series of phenols and anilines as the receptors. Key to the successful development of this sensor system is that coupling the receptors with six different pK_a values with a single fluorophore produces the correct optical properties. This rational design affords a series of optical pH sensors with unique fluorescence property and accurately tunable pH measurement ranging from 1 to 14 pH units. Because of covalent immobilization of the indicators, these sensors demonstrate excellent stability, adequate reversibility, and satisfactory dynamic range up to full pH ranges (pH 1鈥?4).