Concentration Ranges of Antibacterial Cations for Showing the Highest Antibacterial Efficacy but the Least Cytotoxicity against Mammalian Cells: Implications for a New Antibacterial Mechanism

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• 作者：Chengyun Ning ; Xiaolan Wang ; Lihua Li ; Ye Zhu ; Mei Li ; Peng Yu ; Lei Zhou ; Zhengnan Zhou ; Junqi Chen ; Guoxin Tan ; Yu Zhang ; Yingjun Wang ; Chuanbin Mao
• 刊名：Chemical Research in Toxicology
• 出版年：2015
• 出版时间：September 21, 2015
• 年：2015
• 卷：28
• 期：9
• 页码：1815-1822
• 全文大小：527K
• ISSN：1520-5010

文摘

Antibacterial metal ions, such as Ag⁺, Zn²⁺ and Cu²⁺, have been extensively used in medical implants and devices due to their strong broad spectrum of antibacterial activity. However, it is still a controversial issue as to whether they can show the desired antibacterial activity while being toxic to mammalian cells. It is very important to balance their antibacterial effectiveness with minimal damage to mammalian cells. Toward this end, this study is to identify the suitable concentrations of these three ions at which they can effectively kill two types of clinically relevant bacteria (Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)) but show no obvious cytotoxicity on fibroblasts. Such concentration ranges are found to be 2.5 脳 10^鈥? M鈥?0^鈥? M, 10^鈥? M鈥?0^鈥? M, and 10^鈥? M鈥?0^鈥? M for Ag⁺, Zn²⁺, and Cu²⁺, respectively. Investigation of their antibacterial mechanism shows that these three metal ions all show antibacterial property through a mechanism of damaging bacterial cell membranes by the generation of reactive oxygen species but surprisingly preserving the integrity of bacterial genomic DNA. The encouraging results indicate that antibacterial metal ions with controlled concentrations can bring considerable benefits to biomedical applications.