Quencher Group Induced High Specificity Detection of Telomerase in Clear and Bloody Urines by AIEgens

详细信息    查看全文

• 作者：Yuan Zhuang ; Mengshi Zhang ; Bin Chen ; Ruixue Duan ; Xuehong Min ; Zhenyu Zhang ; Fuxin Zheng ; Huageng Liang ; Zujin Zhao ; Xiaoding Lou ; Fan Xia
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：September 15, 2015
• 年：2015
• 卷：87
• 期：18
• 页码：9487-9493
• 全文大小：465K
• ISSN：1520-6882

文摘

Telomerase is a widely used tumor biomarker for early cancer diagnosis. On the basis of the combined use of aggregation-induced emission (AIE) fluorogens and quencher, a quencher group induced high specificity strategy for detection of telomerase activity from cell extracts and cancer patients鈥?urine specimens was creatively developed. In the absence of telomerase, fluorescence background is extremely low due to the short distance between quencher and AIE dye. In the addition of telomerase, fluorescence enhances significantly. The telomerase activity in the E-J, MCF-7, and HeLa extracts equivalent to 5鈥?0鈥?00 cells can be detected by this method in 鈭? h. Furthermore, the distinguishing of telomerase extracted from 38 cancer and 15 normal urine specimens confirms the reliability and practicality of this protocol. In contrast to our previous results (Anal. Chem. 2015, 87, 6822鈥?827), these advanced experiments obtain more remarkable specificity.