Gynura procumbens Reverses Acute and Chronic Ethanol-Induced Liver Steatosis through MAPK/SREBP-1c-Dependent and -Independent Pathways

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• 作者：Xiao-Jun Li ; Yun-Mei Mu ; Ting-Ting Li ; Yan-Ling Yang ; Mei-Tuo Zhang ; Yu-Sang Li ; Wei Kevin Zhang ; He-Bin Tang ; Hong-Cai Shang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：September 30, 2015
• 年：2015
• 卷：63
• 期：38
• 页码：8460-8471
• 全文大小：839K
• ISSN：1520-5118

文摘

The present study aimed to evaluate the hepatoprotective effect and mechanism of action of Gynura procumbens on acute and chronic ethanol-induced liver injuries. Ethanol extract from G. procumbens stems (EEGS) attenuated acute ethanol-induced serum alanine aminotransferase levels and hepatic lipid accumulation. Therefore, EEGS was successively extracted by petroleum, ethyl acetate, and n-butyl alcohol. The results showed that the n-butyl alcohol extract was the active fraction of EEGS, and hence it was further fractionated on a polyamide glass column. The 60% ethanol-eluted fraction that contained 13.6% chlorogenic acid was the most active fraction, and its effect was further evaluated using a chronic model. Both the n-butyl alcohol extract and the 60% ethanol-eluted fraction inhibited chronic ethanol-induced hepatic lipid accumulation by modulating lipid metabolism-related regulators through MAPK/SREBP-1c-dependent and -independent signaling pathways and ameliorated liver steatosis. Our findings suggest that EEGS and one of its active ingredients, chlorogenic acid, may be developed as potential effective agents for ethanol-induced liver injury.