Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction鈥揇erivatization

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• 作者：Mengge Liu ; Guang Chen ; Hailong Guo ; Baolei Fan ; Jianjun Liu ; Qiang Fu ; Xiu Li ; Xiaomin Lu ; Xianen Zhao ; Guoliang Li ; Zhiwei Sun ; Lian Xia ; Shuyun Zhu ; Daoshan Yang ; Ziping Cao ; Hua Wang ; Yourui Suo ; Jinmao You
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：September 16, 2015
• 年：2015
• 卷：63
• 期：36
• 页码：8058-8067
• 全文大小：507K
• ISSN：1520-5118

文摘

Determination of plant growth regulators (PGRs) in a signal transduction system (STS) is significant for transgenic food safety, but may be challenged by poor accuracy and analyte instability. In this work, a microwave-assisted extraction鈥揹erivatization (MAED) method is developed for six acidic PGRs in oil samples, allowing an efficient (<1.5 h) and facile (one step) pretreatment. Accuracies are greatly improved, particularly for gibberellin A₃ (鈭?.72 to 鈭?.65%) as compared with those reported (鈭?2 to 鈭?%). Excellent selectivity and quite low detection limits (0.37鈥?.36 ng mL^鈥?) are enabled by fluorescence detection鈥搈ass spectrum monitoring. Results show the significant differences in acidic PGRs between transgenic and nontransgenic oils, particularly 1-naphthaleneacetic acid (1-NAA), implying the PGRs induced variations of components and genes. This study provides, for the first time, an accurate and efficient determination for labile PGRs involved in STS and a promising concept for objectively evaluating the safety of transgenic foods.