NanoCluster Beacons Enable Detection of a Single N6-Methyladenine

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• 作者：Yu-An Chen ; Judy M. Obliosca ; Yen-Liang Liu ; Cong Liu ; Mary L. Gwozdz ; Hsin-Chih Yeh
• 刊名：Journal of the American Chemical Society
• 出版年：2015
• 出版时间：August 26, 2015
• 年：2015
• 卷：137
• 期：33
• 页码：10476-10479
• 全文大小：383K
• ISSN：1520-5126

文摘

While N⁶-methyladenine (m⁶A) is a common modification in prokaryotic and lower eukaryotic genomes and has many biological functions, there is no simple and cost-effective way to identify a single N⁶-methyladenine in a nucleic acid target. Here we introduce a robust, simple, enzyme-free and hybridization-based method using a new silver cluster probe, termed methyladenine-specific NanoCluster Beacon (maNCB), which can detect single m⁶A in DNA targets based on the fluorescence emission spectra of silver clusters. Not only can maNCB identify m⁶A at the single-base level but it also can quantify the extent of adenine methylation in heterogeneous samples. Our method is superior to high-resolution melting analysis as we can pinpoint the location of m⁶A in the target.