Wide-Scope Screening of Illegal Adulterants in Dietary and Herbal Supplements via Rapid Polarity-Switching and Multistage Accurate Mass Confirmation Using an LC-IT/TOF Hybrid Instrument

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• 作者：Bin Guo ; Meiling Wang ; Yanyan Liu ; Jing Zhou ; Hua Dai ; Zhiqiang Huang ; Lingling Shen ; Qingsheng Zhang ; Bo Chen
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：August 12, 2015
• 年：2015
• 卷：63
• 期：31
• 页码：6954-6967
• 全文大小：894K
• ISSN：1520-5118

文摘

A new analytical strategy was developed that integrates a generic sample preparation into a liquid chromatography鈥搈ultistage ion trap/time-of-flight mass spectrometry (LC-IT(MSⁿ)/TOF), allowing for large-scale screening and qualitative confirmation of wide-scope illegal adulterants in different food matrices. Samples were pretreated by a fast single-tube multifunction extraction for accurate multistage mass measurement on the hybrid LC-IT/TOF system. A qualitative validation performed for over 500 analyte鈥搈atrix pairs showed the method can reduce most of the matrix effects and achieve a lower limit of confirmation at 0.1 mg/kg for 73% of the target compounds. A unique combination of dual-polarity detection, retention time, isotopic profile, and accurate MSⁿ spectra enables more comprehensive and precise confirmation, based on the multiparameter matching by automated library searching against the user-created database. Finally, the applicability of this LC-IT(MSⁿ)/TOF-based screening procedure for discriminating coeluting isobars, identifying nontarget adulterants, and even tentatively elucidating unexpected species in real samples is demonstrated.