Stereoselective Determination of Tebuconazole in Water and Zebrafish by Supercritical Fluid Chromatography Tandem Mass Spectrometry

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• 作者：Na Liu ; Fengshou Dong ; Jun Xu ; Xingang Liu ; Zenglong Chen ; Yan Tao ; Xinglu Pan ; XiXi Chen ; Yongquan Zheng
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：July 22, 2015
• 年：2015
• 卷：63
• 期：28
• 页码：6297-6303
• 全文大小：352K
• ISSN：1520-5118

文摘

A simple and sensitive method for the enantioselective determination of tebuconazole enantiomers in water and zebrafish has been established using supercritical fluid chromatography (SFC)-MS/MS. The effects of the chiral stationary phases, mobile phase, auto back pressure regulator (ABPR) pressure, column temperature, flow rate of the mobile phase, and compensation pump solvent were evaluated. Finally, the optimal SFC-MS/MS working conditions were determined to include a CO₂/MeOH mobile phase (87:13, v/v), 2.0 mL/min flow rate, 2200 psi ABPR, and 30 掳C column temperature using a Chiralpak IA-3 chiral column under electrospray ionization positive mode. The modified QuEChERS method was applied to water and zebrafish samples. The mean recoveries for the tebuconazole enantiomers were 79.8鈥?08.4% with RSDs 鈮?7.0% in both matrices. The LOQs ranged from 0.24 to 1.20 渭g/kg. The developed analytical method was further validated by application to the analysis of authentic samples.