TiO2 Nanoparticle Exposure Decreases Spermatogenesis via Biochemical Dysfunctions in the Testis of Male Mice

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• 作者：Fashui Hong ; Wenhui Si ; Xiaoyang Zhao ; Ling Wang ; Yingjun Zhou ; Ming Chen ; Yushaung Ge ; Qi Zhang ; Yajing Wang ; Jianhao Zhang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：August 12, 2015
• 年：2015
• 卷：63
• 期：31
• 页码：7084-7092
• 全文大小：471K
• ISSN：1520-5118

文摘

TiO₂ nanoparticles (NPs) have been demonstrated to suppress spermatogenesis in animals, while there is little data related to the biochemical dysfunctions during spermatogenesis due to exposure to TiO₂ NPs. In this study, male mice have been exposed to TiO₂ NPs via intragastric administration for 60 consecutive days. The findings showed that TiO₂ NP exposure resulted in lesions of testis and epididymis, deductions in sperm concentration and sperm motility, and an increase of the number of abnormal sperm in mice. Furthermore, TiO₂ NP exposure with 2.5, 5, or 10 mg/kgbw decreased activities of lactate dehydrogenase (鈭?1.59% to 鈭?9.84%), sorbitol dehydrogenase (鈭?3.56% to 鈭?7.33%), succinate dehydrogenase (鈭?7.04% to 鈭?7.85%), glucose-6-phosphate dehydrogenase (鈭?8.3% to 鈭?6.42%), Na⁺/K⁺-ATPase (鈭?5.59% to 鈭?3.11%), Ca²⁺-ATPase (鈭?2.44% to 鈭?5.41%), and Ca²⁺/Mg²⁺-ATPase (鈭?8.25% to 鈭?5.72%), and elevated activities of acid phosphatase (+10.48% to +40.0%), alkaline phosphatase (+20.65% to +64.07%), and total nitric oxide synthase (+0.68- to +2.3-fold) in the testes of mice, respectively. In addition, TiO₂ NP exposure caused excessive production of reactive oxygen species (+16.15% to +110.62%), and increased malondialdehyde of lipid peroxidation product (+38.96% to +118.07%), carbonyl of protein oxidative product (+20.98% to +108.1%), and 8-hydroxydeoxyguanosine of DNA oxidative product (+0.9- to +1.83-fold) in the testes, respectively. It implied that spermatogenesis suppression caused by TiO₂ NP exposure may be associated with alterations of testicular marked enzymes and oxidative stress in the testes.