Metabolomic Analysis Reveals Cyanidins in Black Raspberry as Candidates for Suppression of Lipopolysaccharide-Induced Inflammation in Murine Macrophages

详细信息    查看全文

• 作者：Young-Hee Jo ; Hyun-Chang Park ; Seulgi Choi ; Sugyeong Kim ; Cheng Bao ; Hyung Woo Kim ; Hyung-Kyoon Choi ; Hong Jin Lee ; Joong-Hyuck Auh
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：June 10, 2015
• 年：2015
• 卷：63
• 期：22
• 页码：5449-5458
• 全文大小：634K
• ISSN：1520-5118

文摘

The extracts produced by multisolvent extraction and subfractionation with preparative liquid chromatography of black raspberry (Rubus coreanus Miquel) cultivated in Gochang, South Korea, were tested for their anti-inflammatory effects. The metabolomic profiling and analysis by orthogonal partial least-squares discriminant analysis (OLPS-DA) suggested that cyanidin, cyanidin-3-glucoside (C3G), and cyanidin-3-rutinoside (C3R) were key components for the anti-inflammatory responses in the most active fraction BF3-1, where they were present at 0.44, 1.26, and 0.56 渭g/mg of BF3-1, respectively. Both BF3-1 and mixture of these cyanidins at the same ratio reduced lipopolysaccharide (LPS)-induced protein level of iNOS expression and suppressed mRNA and protein expressions of tumor necrosis factor (TNF)-伪, interleukin (IL)-6, and IL-1尾 through inhibiting the phosphorylation of mitogen-activated protein kinases (MAPKs) and STAT3 in murine macrophage RAW264.7 cells. Overall, the results suggested that co-administration of cyanidin, C3G, and C3R is more effective than that of cyanidin alone and that the coexistence of these anthocyanin components in black raspberry plays a vital role in regulating LPS-induced inflammation even at submicromolar concentrations, making it possible to explain the health beneficial activity of its extracts.