Antifungal, Phytotoxic, and Cytotoxic Activities of Metabolites from Epichlo毛 bromicola, a Fungus Obtained from Elymus tangutorum Grass

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• 作者：Qiu-Yan Song ; Zhi-Biao Nan ; Kun Gao ; Hui Song ; Pei Tian ; Xing-Xu Zhang ; Chun-Jie Li ; Wen-Bo Xu ; Xiu-Zhang Li
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：October 14, 2015
• 年：2015
• 卷：63
• 期：40
• 页码：8787-8792
• 全文大小：291K
• ISSN：1520-5118

文摘

The development of high-quality herbage is an important aspect of animal husbandry. Inoculating beneficial fungi onto inferior grass is a feasible strategy for producing new varieties of high-quality herbage. Epichlo毛 bromicola is a candidate fungus that is isolated from Elymus tangutorum. A total of 17 metabolites, 1鈥?b>17, were obtained from E. bromicola, and their biological activities were assayed. Metabolite 1 exhibited antifungal activities against Alternaria alternata, Fusarium avenaceum, Bipolaris sorokiniana, and Curvularia lunata. EC₅₀ values ranged from 0.7 to 5.3 渭M, which were better than the positive control, chlorothalonil. Metabolite 8 displayed obvious phytotoxic effects toward Lolium perenne and Poa crymophila seedlings, and it was as active as glyphosate. None of these isolated metabolites displayed cytotoxicity against Madin-Darby bovine kidney cells. The IC₅₀ values were greater than 100 渭M, and the metabolites increased the growth of the cells at a concentration of 12.5 渭M. The bioassay indicated that E. bromicola may be a beneficial fungus for producing new varieties of herbage with various resistances. Additionally, metabolite 7, 3-(2鈥?(4鈥?hydroxyphenyl)acetoxy)-2S-methylpropanoic acid, is a new natural product, and its stereochemistry was determined by means of optical rotation computation and chemical reactions.