Tissue-Based Proteogenomics Reveals that Human Testis Endows Plentiful Missing Proteins

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• 作者：Yao Zhang ; Qidan Li ; Feilin Wu ; Ruo Zhou ; Yingzi Qi ; Na Su ; Lingsheng Chen ; Shaohang Xu ; Tao Jiang ; Chengpu Zhang ; Gang Cheng ; Xinguo Chen ; Degang Kong ; Yujia Wang ; Tao Zhang ; Jin Zi ; Wei Wei ; Yuan Gao ; Bei Zhen ; Zhi Xiong ; Songfeng Wu ; Pengyuan Yang ; Quanhui Wang ; Bo Wen ; Fuchu He ; Ping Xu ; Siqi Liu
• 刊名：Journal of Proteome Research
• 出版年：2015
• 出版时间：September 4, 2015
• 年：2015
• 卷：14
• 期：9
• 页码：3583-3594
• 全文大小：772K
• ISSN：1535-3907

文摘

Investigations of missing proteins (MPs) are being endorsed by many bioanalytical strategies. We proposed that proteogenomics of testis tissue was a feasible approach to identify more MPs because testis tissues have higher gene expression levels. Here we combined proteomics and transcriptomics to survey gene expression in human testis tissues from three post-mortem individuals. Proteins were extracted and separated with glycine- and tricine-SDS-PAGE. A total of 9597 protein groups were identified; of these, 166 protein groups were listed as MPs, including 138 groups (83.1%) with transcriptional evidence. A total of 2948 proteins are designated as MPs, and 5.6% of these were identified in this study. The high incidence of MPs in testis tissue indicates that this is a rich resource for MPs. Functional category analysis revealed that the biological processes that testis MPs are mainly involved in are sexual reproduction and spermatogenesis. Some of the MPs are potentially involved in tumorgenesis in other tissues. Therefore, this proteogenomics analysis of individual testis tissues provides convincing evidence of the discovery of MPs. All mass spectrometry data from this study have been deposited in the ProteomeXchange (data set identifier PXD002179).