Quest for Missing Proteins: Update 2015 on Chromosome-Centric Human Proteome Project

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• 作者：P茅ter Horvatovich ; Emma K. Lundberg ; Yu-Ju Chen ; Ting-Yi Sung ; Fuchu He ; Edouard C. Nice ; Robert J. Goode ; Simon Yu ; Shoba Ranganathan ; Mark S. Baker ; Gilberto B. Domont ; Erika Velasquez ; Dong Li ; Siqi Liu ; Quanhui Wang ; Qing-Yu He ; Rajasree Menon ; Yuanfang Guan ; Fernando J. Corrales ; Victor Segura ; J. Ignacio Casal ; Alberto Pascual-Montano ; Juan P. Albar ; Manuel Fuentes ; Maria Gonzalez-Gonzalez ; Paula Diez ; Nieves Ibarrola ; Rosa M. Degano ; Yassene Mohammed ; Christoph H. Borchers ; Andrea Urbani ; Alessio Soggiu ; Tadashi Yamamoto ; Ghasem Hosseini Salekdeh ; Alexander Archakov ; Elena Ponomarenko ; Andrey Lisitsa ; Cheryl F. Lichti ; Ekaterina Mostovenko ; Roger A. Kroes ; Melinda Rezeli ; 脕kos V茅gv谩ri ; Thomas E. Fehniger ; Rainer Bischoff ; Juan Antonio Vizca铆no ; Eric W. Deutsch ; Lydie Lane ; Carol L. Nilsson ; Gy枚rgy Marko-Varga ; Gilbert S. Omenn ; Seul-Ki Jeong ; Jong-Sun Lim ; Young-Ki Paik ; William S. Hancock
• 刊名：Journal of Proteome Research
• 出版年：2015
• 出版时间：September 4, 2015
• 年：2015
• 卷：14
• 期：9
• 页码：3415-3431
• 全文大小：607K
• ISSN：1535-3907

文摘

This paper summarizes the recent activities of the Chromosome-Centric Human Proteome Project (C-HPP) consortium, which develops new technologies to identify yet-to-be annotated proteins (termed 鈥渕issing proteins鈥? in biological samples that lack sufficient experimental evidence at the protein level for confident protein identification. The C-HPP also aims to identify new protein forms that may be caused by genetic variability, post-translational modifications, and alternative splicing. Proteogenomic data integration forms the basis of the C-HPP鈥檚 activities; therefore, we have summarized some of the key approaches and their roles in the project. We present new analytical technologies that improve the chemical space and lower detection limits coupled to bioinformatics tools and some publicly available resources that can be used to improve data analysis or support the development of analytical assays. Most of this paper鈥檚 content has been compiled from posters, slides, and discussions presented in the series of C-HPP workshops held during 2014. All data (posters, presentations) used are available at the C-HPP Wiki (http://c-hpp.webhosting.rug.nl/) and in the Supporting Information.