Optical Properties of 2-Methacryloyloxyethyl Phosphorylcholine-Protected Au4 Nanoclusters and Their Fluorescence Sensing of C-Reactive Protein

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• 作者：Junya Yoshimoto ; Arunee Sangsuwan ; Issey Osaka ; Kazuko Yamashita ; Yasuhiko Iwasaki ; Mitsuru Inada ; Ryuichi Arakawa ; Hideya Kawasaki
• 刊名：Journal of Physical Chemistry C
• 出版年：2015
• 出版时间：June 25, 2015
• 年：2015
• 卷：119
• 期：25
• 页码：14319-14325
• 全文大小：334K
• ISSN：1932-7455

文摘

We present the solution synthesis of thiolated 2-methacryloyloxyethyl phosphorylcholine (MPC)-protected Au nanoclusters (NCs). This water-soluble lipid-mimetic MPC was first used for the size focusing synthesis of thiolate (SR)-protected Au_n(SR)_m NCs. Au₂₅(MPC)₁₈ and Au₄(MPC)₄ NCs are selectively synthesized, without the need for electrophoretic or chromatographic isolation of size mixed products, by including ethanol or not in the solvent. The Au₄(MPC)₄ NCs emit at yellow wavelengths (580鈥?00 nm) with a quantum yield (3.6%) and an average lifetime of 1.5 渭s. Also for the first time, we report C-reactive protein (CRP) sensing using Au NCs, with a detection limit (5 nM) low enough for the clinical diagnosis of inflammation. This is based on the quenching effect of specific CRP鈥揗PC interactions on the fluorescence of the Au₄(MPC)₄ NCs.