A Novel Poly(ionic liquid) Interface-Free Two-Dimensional Monolithic Material for the Separation of Multiple Types of Glycoproteins

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• 作者：Cuicui Liu ; Qiliang Deng ; Guozhen Fang ; Xuan Huang ; Shuo Wang ; Jinsong He
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：September 16, 2015
• 年：2015
• 卷：7
• 期：36
• 页码：20430-20437
• 全文大小：441K
• ISSN：1944-8252

文摘

Currently, many types of affinity materials have been developed for the enrichment of glycoproteins potentially considered to be clinical biomarkers; however, they can not effectively distinguish between different glycoproteins and thus lack the functionality that may be the key to the diagnosis of specific diseases. In the present work, a novel interface-free 2D monolithic material has been developed for the separation of multiple types of glycoproteins, in which boronate-functionalized graphene acts as preconcentration segment and poly(guanidinium ionic liquid) acts as separation segment. The resultant 2D material was characterized by X-ray photoelectron spectroscopy, elemental analysis, and electroosmotic flow analysis to demonstrate successful modification at each step. The performance of this 2D material was evaluated by capillary electrochromatography and allowed the successful online concentration and separation of five standard glycoproteins. The high separation efficiency can be largely attributed to the good orthogonality of boronate-functionalized graphene monolith and poly(guanidinium ionic liquid) monolith.