Highly Sensitive Electrochemical Biosensor for Evaluation of Oxidative Stress Based on the Nanointerface of Graphene Nanocomposites Blended with Gold, Fe3O4, and Platinum Nanopar

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• 作者：Le Wang ; Yuanyuan Zhang ; Chuansheng Cheng ; Xiaoli Liu ; Hui Jiang ; Xuemei Wang
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：August 26, 2015
• 年：2015
• 卷：7
• 期：33
• 页码：18441-18449
• 全文大小：521K
• ISSN：1944-8252

文摘

High levels of H₂O₂ pertain to high oxidative stress and are associated with cancer, autoimmune, and neurodegenerative disease, and other related diseases. In this study, a sensitive H₂O₂ biosensor for evaluation of oxidative stress was fabricated on the basis of the reduced graphene oxide (RGO) nanocomposites decorated with Au, Fe₃O₄, and Pt nanoparticles (RGO/AuFe₃O₄/Pt) modified glassy carbon electrode (GCE) and used to detect the released H₂O₂ from cancer cells and assess the oxidative stress elicited from H₂O₂ in living cells. Electrochemical behavior of RGO/AuFe₃O₄/Pt nanocomposites exhibits excellent catalytic activity toward the relevant reduction with high selection and sensitivity, low overpotential of 0 V, low detection limit of 鈭?.1 渭M, large linear range from 0.5 渭M to 11.5 mM, and outstanding reproducibility. The as-prepared biosensor was applied in the measurement of efflux of H₂O₂ from living cells including healthy normal cells and tumor cells under the external stimulation. The results display that this new nanocomposites-based biosensor is a promising candidate of nonenzymatic H₂O₂ sensor which has the possibility of application in clinical diagnostics to assess oxidative stress of different kinds of living cells.