Light-Directed Synthesis of High-Density Peptide Nucleic Acid Microarrays

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• 作者：Feipeng Yang ; Bo Dong ; Kaixuan Nie ; Huanhuan Shi ; Yanqi Wu ; Hongyin Wang ; Zhengchun Liu
• 刊名：ACS Combinatorial Science
• 出版年：2015
• 出版时间：October 12, 2015
• 年：2015
• 卷：17
• 期：10
• 页码：608-614
• 全文大小：367K
• ISSN：2156-8944

文摘

Peptide nucleic acids (PNAs) are a class of nucleic acid mimics that can bind to the complementary DNA or RNA with high specificity and sensitivity. PNA-based microarrays have distinct characteristics and have improved performance in many aspects compared to DNA microarrays. A new set of PNA monomers has been synthesized and used as the building blocks for the preparation of high density PNA microarrays. These monomers have their backbones protected by the photolabile group 2-(2-nitrophenyl)propyloxy carbonyl (NPPOC), and their exocyclic amino groups protected by amide carbonyl groups. A light-directed synthesis system was designed and applied to the in situ synthesis of a PNA microarray with a density of over 10鈥?00 probes per square centimeter. This PNA microarray was able to detect single and multiple base-mismatches correctly with a high discrimination ratio.