Mussel-Inspired Artificial Grafts for Functional Ligament Reconstruction

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• 作者：Hong Li ; Shiyi Chen ; Jiwu Chen ; Jiang Chang ; Mengchi Xu ; Yaying Sun ; Chengtie Wu
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：July 15, 2015
• 年：2015
• 卷：7
• 期：27
• 页码：14708-14719
• 全文大小：688K
• ISSN：1944-8252

文摘

The development of an artificial graft with distinct osteogenetic activity to enhance osseointegration and to induce the formation of biomimetic tissue structure for ligament reconstruction remains a significant challenge. Inspired by mussels, biomimetic calcium phosphate apatite/polydopamine hybridized鈥損olyethylene terephthalate (APA/PDA鈥揚ET) grafts were successfully prepared. The efficacy and mechanism of APA/PDA鈥揚ET grafts to induce osseointegration were systematically investigated. The results from the in vitro study indicated that the prepared APA/PDA鈥揚ET grafts support the attachment of bone marrow stromal cells (BMSCs) and stimulate the proliferation and osteogenic/angiogenic differentiation of BMSCs via activation of the PKC/p-ERK1/2 signaling pathway. In vivo, histological and radiological results further demonstrate that the APA/PDA鈥揚ET grafts significantly improve osseointegration by inducing the formation of new bone tissue and the fibrocartilage transitional zone compared with pure PET grafts. In addition, the pull-out strength of the APA/PDA鈥揚ET grafts is significantly higher than that of the pure PET grafts 12 weeks after surgery. These results suggest that this mussel-inspired biomimetic method is an effective strategy for modifying artificial grafts, and the prepared APA/PDA鈥揚ET grafts, which possess a beneficial interface, can significantly improve in vivo osseointegration for ligament reconstruction via the synergistic effect of polydopamine and apatite.