Serially Ordered Magnetization of Nanoclusters via Control of Various Transition Metal Dopants for the Multifractionation of Cells in Microfluidic Magnetophoresis Devices

详细信息    查看全文

• 作者：Byunghoon Kang ; Bumjoon Cha ; Bongsoo Kim ; Seungmin Han ; Moo-Kwang Shin ; Eunji Jang ; Hyun-Ouk Kim ; Seo Ryung Bae ; Unyong Jeong ; Il Moon ; Hye yeong Son ; Yong-Min Huh ; Seungjoo Haam
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：January 19, 2016
• 年：2016
• 卷：88
• 期：2
• 页码：1078-1082
• 全文大小：319K
• ISSN：1520-6882

文摘

A novel method (i.e., continuous magnetic cell separation in a microfluidic channel) is demonstrated to be capable of inducing multifractionation of mixed cell suspensions into multiple outlet fractions. Here, multicomponent cell separation is performed with three different distinguishable magnetic nanoclusters (MnFe₂O₄, Fe₃O₄, and CoFe₂O₄), which are tagged on A431 cells. Because of their mass magnetizations, which can be ideally altered by doping with magnetic atom compositions (Mn, Fe, and Co), the trajectories of cells with each magnetic nanocluster in a flow are shown to be distinct when dragged under the same external magnetic field; the rest of the magnetic characteristics of the nanoclusters are identically fixed. This proof of concept study, which utilizes the magnetization-controlled nanoclusters (NCs), suggests that precise and effective multifractionation is achievable with high-throughput and systematic accuracy for dynamic cell separation.