Chip-Based Magnetic Solid-Phase Microextraction Online Coupled with MicroHPLC–ICPMS for the Determination of Mercury Species in Cells

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• 作者：Han Wang ; Beibei Chen ; Siqi Zhu ; Xiaoxiao Yu ; Man He ; Bin Hu
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：January 5, 2016
• 年：2016
• 卷：88
• 期：1
• 页码：796-802
• 全文大小：316K
• ISSN：1520-6882

文摘

Trace mercury speciation in cells is critical to understand its cytotoxicity and cell protection mechanism. In this work, we fabricated a chip-based magnetic solid-phase microextraction (MSPME) system, integrating a cell lysis unit as well as a sample extraction unit, and online combined it with micro high-performance liquid chromatography (microHPLC)–inductively coupled plasma mass spectrometry (ICPMS) for the speciation of mercury in HepG2 cells. Magnetic nanoparticles with sulfhydryl functional group were synthesized and self-assembled in the microchannels for the preconcentration of mercury species in cells under an external magnetic field. The enrichment factors are ca. 10-fold, and the recoveries for the spiked samples are in the range of 98.3–106.5%. The developed method was used to analyze target mercury species in Hg²⁺ or MeHg⁺ incubated HepG2 cells. The results demonstrated that MeHg⁺ entered into the HepG2 cells more easily than Hg²⁺, and part of the MeHg⁺ might demethylate into Hg²⁺ in HepG2 cells. Besides, comprehensive speciation of mercury in incubated cells revealed different detoxification mechanisms of Hg²⁺ and MeHg⁺ in Hg²⁺ or MeHg⁺ incubated HepG2 cells.