Construction of a Biomimetic Surface on Microfluidic Chips for Biofouling Resistance

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• 作者：Hongyan Bi ; Wei Zhong ; Sheng Meng ; Jilie Kong ; Pengyuan Yang ; and Baohong Liu
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：May 15, 2006
• 年：2006
• 卷：78
• 期：10
• 页码：3399 - 3405
• 全文大小：220K
• 年卷期：v.78,no.10(May 15, 2006)
• ISSN：1520-6882

文摘

A biomimetic surface has been formed on the poly(methylmethacrylate) (PMMA) microfluidic chips for biofoulingresistance on the basis of a simple modification. Accordingly, an amphiphilic phospholipid copolymer of 2-methacryloyloxyethyl phosphorylcholine and n-butyl methacrylate (PMB) was developed to introduce the phosphorylcholine functional groups onto the PMMA surfacevia the anchoring of hydrophobic n-butyl methacrylateunits. The 2-methacryloyloxyethyl phosphorylcholine segments could form hydrophilic domains, considered to belocated on the surface, to provide a biocompatible surface.X-ray photoelectron spectroscopy and Fourier transforminfrared spectra confirmed the success of surface functionalization. The PMB-modified microchips containingphosphorylcholine moieties exhibited more stable electroosmotic mobility compared with the untreated one. Inaddition to being characterized for minimized nonspecificadhesion of serum proteins and plasma platelets, thePMB-functionalized microchannels have been exemplifiedby electrophoresis of proteins. This one-step procedureoffers an effective approach for a biomimetic surfacedesign on microfluidic chips, which is promising in high-throughput and complex biological analysis.