Calcium Plasma Implanted Titanium Surface with Hierarchical Microstructure for Improving the Bone Formation

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• 作者：Mengqi Cheng ; Yuqin Qiao ; Qi Wang ; Guodong Jin ; Hui Qin ; Yaochao Zhao ; Xiaochun Peng ; Xianlong Zhang ; Xuanyong Liu
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：June 17, 2015
• 年：2015
• 卷：7
• 期：23
• 页码：13053-13061
• 全文大小：618K
• ISSN：1944-8252

文摘

Introducing hierarchical microstructure and bioactive trace elements simultaneously onto the surface of titanium implant is a very effective way to improve the osseointegration between bone and implant. In this work, hierarchical topography was prepared on Ti surface via acid etching and sandblasting (SLA) to form micropits and microcavities then underwent Ca plasma immersion ion implantation (Ca鈥揚III) process. The surface wettability and roughness did not change obviously before and after Ca鈥揚III process. The in vitro evaluations including cell adhesion, activity, alkaline phosphatase (ALP), osteogenic genes (Runx2, OSX, ALP, BSP, Col1a1, OPN, and OC), and protein (BSP, Col1a1, OPN, and OC) expressions revealed that the introduction of Ca ions onto the surface of SLA-treated Ti can promote greater osteoblasts adhesion, spread and proliferation, which in return further accelerated the maturation and mineralization of osteoblasts. More importantly, in vivo evaluations including Micro-CT evaluation, histological observations, push-out test, sequential fluorescent labeling and histological observations verified that Ca-SLA-treated Ti implants could efficiently promote new bone formation in early times. These promising results suggest that Ca-SLA-treated Ti has the potential for future application in orthopedic field.