Sensitive Detection of Organophosphorus Pesticides in Medicinal Plants Using Ultrasound-Assisted Dispersive Liquid–Liquid Microextraction Combined with Sweeping Micellar Electrokinetic Chromatography

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• 作者：Jin-Chao Wei ; Ji Hu ; Ji-Liang Cao ; Jian-Bo Wan ; Cheng-Wei He ; Yuan-Jia Hu ; Hao Hu ; Peng Li
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：February 3, 2016
• 年：2016
• 卷：64
• 期：4
• 页码：932-940
• 全文大小：392K
• ISSN：1520-5118

文摘

A simple, rapid, and sensitive method using ultrasound-assisted dispersive liquid–liquid microextraction (UA-DLLME) combined with sweeping micellar electrokinetic chromatography (sweeping-MEKC) has been developed for the determination of nine organophosphorus pesticides (chlorfenvinphos, parathion, quinalphos, fenitrothion, azinphos-ethyl, parathion-methyl, fensulfothion, methidathion, and paraoxon). The important parameters that affect the UA-DLLME and sweeping efficiency were investigated. Under the optimized conditions, the proposed method provided 779.0–6203.5-fold enrichment of the nine pesticides compared to the normal MEKC method. The limits of detection ranged from 0.002 to 0.008 mg kg^–1. The relative standard deviations of the peak area ranged from 1.2 to 6.5%, indicating the good repeatability of the method. Finally, the developed UA-DLLME–sweeping-MEKC method has been successfully applied to the analysis of the investigated pesticides in several medicinal plants, including Lycium chinense, Dioscorea opposite, Codonopsis pilosula, and Panax ginseng, indicating that this method is suitable for the determination of trace pesticide residues in real samples with complex matrices.