B Cells Loaded with Synthetic Particulate Antigens: A Versatile Platform To Generate Antigen-Specific Helper T Cells for Cell Therapy

详细信息    查看全文

• 作者：Antoine Sicard ; Alice Koenig ; Stéphanie Graff-Dubois ; Sébastien Dussurgey ; Angéline Rouers ; Valérie Dubois ; Pascal Blanc ; Dimitri Chartoire ; Elisabeth Errazuriz-Cerda ; Helena Paidassi ; Morgan Taillardet ; Emmanuel Morelon ; Arnaud Moris ; Thierry Defrance ; Olivier Thaunat
• 刊名：Nano Letters
• 出版年：2016
• 出版时间：January 13, 2016
• 年：2016
• 卷：16
• 期：1
• 页码：297-308
• 全文大小：800K
• ISSN：1530-6992

文摘

Adoptive cell therapy represents a promising approach for several chronic diseases. This study describes an innovative strategy for biofunctionalization of nanoparticles, allowing the generation of synthetic particulate antigens (SPAg). SPAg activate polyclonal B cells and vectorize noncognate proteins into their endosomes, generating highly efficient stimulators for ex vivo expansion of antigen-specific CD4+ T cells. This method also allows harnessing the ability of B cells to polarize CD4+ T cells into effectors or regulators.