Analysis of Carbohydrate–Carbohydrate Interactions Using Sugar-Functionalized Silicon Nanoparticles for Cell Imaging

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• 作者：Chian-Hui Lai ; Julia Hütter ; Chien-Wei Hsu ; Hidenori Tanaka ; Silvia Varela-Aramburu ; Luisa De Cola ; Bernd Lepenies ; Peter H. Seeberger
• 刊名：Nano Letters
• 出版年：2016
• 出版时间：January 13, 2016
• 年：2016
• 卷：16
• 期：1
• 页码：807-811
• 全文大小：351K
• ISSN：1530-6992

文摘

Protein-carbohydrate binding depends on multivalent ligand display that is even more important for low affinity carbohydrate–carbohydrate interactions. Detection and analysis of these low affinity multivalent binding events are technically challenging. We describe the synthesis of dual-fluorescent sugar-capped silicon nanoparticles that proved to be an attractive tool for the analysis of low affinity interactions. These ultrasmall NPs with sizes of around 4 nm can be used for NMR quantification of coupled sugars. The silicon nanoparticles are employed to measure the interaction between the cancer-associated glycosphingolipids GM3 and Gg3 and the associated k_D value by surface plasmon resonance experiments. Cell binding studies, to investigate the biological relevance of these carbohydrate–carbohydrate interactions, also benefit from these fluorescent sugar-capped nanoparticles.