A Supramolecular Sensor Array Using Lanthanide-Doped Nanoparticles for Sensitive Detection of Glyphosate and Proteins

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• 作者：Meng Wang ; Hebo Ye ; Lei You ; Xueyuan Chen
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：January 13, 2016
• 年：2016
• 卷：8
• 期：1
• 页码：574-581
• 全文大小：500K
• ISSN：1944-8252

文摘

Lanthanide (Ln³⁺)-doped nanoparticles (NPs) are an intensive area of research in chemical and materials sciences. Herein a sensor array of Ln³⁺-doped NPs was developed for the first time toward sensitive molecular sensing based on a novel strategy of the hybridized time-resolved F?rster resonance energy transfer (TR-FRET) with the indicator displacement assay (IDA) concept (TR-FRET-IDA). The sensor platform was generated in situ by binding a series of negatively charged indicators on the surface of ligand-free LiYF₄:Ce/Tb NPs. The TR-FRET between NPs and dyes resulted in indicator emission and was employed as a means of removing undesired short-lived background luminescence from the indicator effectively. Displacement of indicators from the NP/indicator ensembles by glyphosate, a common herbicide, led to turn-off of the indicator emission. The sensor array was able to successfully discriminate 11 biologically relevant anions with high accuracy and sensitivity in pure aqueous buffer both qualitatively and quantitatively. Furthermore, the differentiation of six model proteins in the nM range was achieved with 100% accuracy for the classification, thereby demonstrating the versatility of this simple sensor platform. The study of the mechanism of binding and signal modulation further verified TR-FRET-IDA as a reliable sensing paradigm.