Synthesis of Thermoresponsive Amphiphilic Polyurethane Gel as a New Cell Printing Material near Body Temperature

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• 作者：Yi-Chun Tsai ; Suming Li ; Shiaw-Guang Hu ; Wen-Chi Chang ; U-Ser Jeng ; Shan-hui Hsu
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：December 23, 2015
• 年：2015
• 卷：7
• 期：50
• 页码：27613-27623
• 全文大小：634K
• ISSN：1944-8252

文摘

Waterborne polyurethane (PU) based on poly(ε-caprolactone) (PCL) diol and a second oligodiol containing amphiphilic blocks was synthesized in this study. The microstructure was characterized by dynamic light scattering (DLS), small-angle X-ray scattering (SAXS), and rheological measurement of the PU dispersion. The surface hydrophilicity measurement, infrared spectroscopy, wide-angle X-ray diffraction, mechanical and thermal analyses were conducted in solid state. It was observed that the presence of a small amount of amphiphilic blocks in the soft segment resulted in significant changes in microstructure. When 90 mol % PCL diol and 10 mol % amphiphilic blocks of poly(l-lactide)–poly(ethylene oxide) (PLLA–PEO) diol were used as the soft segment, the synthesized PU had a water contact angle of ～24° and degree of crystallinity of ～14%. The dispersion had a low viscosity below room temperature. As the temperature was raised to body temperature (37 °C), the dispersion rapidly (～170 s) underwent sol–gel transition with excellent gel modulus (G′ ≈ 6.5 kPa) in 20 min. PU dispersions with a solid content of 25–30% could be easily mixed with cells in sol state, extruded by a 3D printer, and deposited layer by layer as a gel. Cells remained alive and proliferating in the printed hydrogel scaffold. We expect that the development of novel thermoresponsive PU system can be used as smart injectable hydrogel and applied as a new type of bio-3D printing ink.