Synthesis, Characterization, and Application of Core–Shell Co0.16Fe2.84O4@NaYF4(Yb, Er) and Fe3O4@NaYF4(Yb, Tm) Nanoparticle as Trimodal (MRI, PET/SPECT, and Optical) Imaging Agents

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• 作者：Xianjin Cui ; Domokos Mathe ; Noémi Kovács ; Ildikó Horváth ; Maite Jauregui-Osoro ; Rafael Torres Martin de Rosales ; Gregory E. D. Mullen ; Wilson Wong ; Yong Yan ; Dirk Krüger ; Andrei N. Khlobystov ; Maria Gimenez-Lopez ; Mariann Semjeni ; Krisztián Szigeti ; Dániel S Veres ; Haizhou Lu ; Ignacio Hernández ; William P. Gillin ; Andrea Protti ; Katalin Kis Petik ; Mark A. Green ; Philip J. Blower
• 刊名：Bioconjugate Chemistry
• 出版年：2016
• 出版时间：February 17, 2016
• 年：2016
• 卷：27
• 期：2
• 页码：319-328
• 全文大小：505K
• ISSN：1520-4812

文摘

Multimodal nanoparticulate materials are described, offering magnetic, radionuclide, and fluorescent imaging capabilities to exploit the complementary advantages of magnetic resonance imaging (MRI), positron emission tomography/single-photon emission commuted tomography (PET/SPECT), and optical imaging. They comprise Fe₃O₄@NaYF₄ core/shell nanoparticles (NPs) with different cation dopants in the shell or core, including Co_0.16Fe_2.84O₄@NaYF₄(Yb, Er) and Fe₃O₄@NaYF₄(Yb, Tm). These NPs are stabilized by bisphosphonate polyethylene glycol conjugates (BP-PEG), and then show a high transverse relaxivity (r₂) up to 326 mMp>–1p> sp>–1p> at 3T, a high affinity to [p>18p>F]-fluoride or radiometal-bisphosphonate conjugates (e.g., p>64p>Cu and p>99mp>Tc), and fluorescent emissions from 500 to 800 nm under excitation at 980 nm. The biodistribution of intravenously administered particles determined by PET/MR imaging suggests that negatively charged Co_0.16Fe_2.84O₄@NaYF₄(Yb, Er)-BP-PEG (10K) NPs cleared from the blood pool more slowly than positively charged NPs Fe₃O₄@NaYF₄(Yb, Tm)-BP-PEG (2K). Preliminary results in sentinel lymph node imaging in mice indicate the advantages of multimodal imaging.