Radical Pair-Driven Luminescence of Quantum Dots for Specific Detection of Peroxynitrite in Living Cells

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• 作者：Wenjuan Zhou ; Yuqing Cao ; Dandan Sui ; Chao Lu
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：March 1, 2016
• 年：2016
• 卷：88
• 期：5
• 页码：2659-2665
• 全文大小：387K
• ISSN：1520-6882

文摘

There is currently great interest in developing chemiluminescence (CL) probes that can selectively detect peroxynitrite (ONOO^–) in living cells. In comparison with other reactive oxygen species (ROS), ONOO^– can spontaneously decompose into a series of radicals. Notably, the interaction of quantum dots (QDs) with oxidizing/reducing ROS radicals can generate a strong CL emission by electron-transfer annihilation. Herein, we report a novel CL probe that affords the ability to distinguish ONOO^– from other ROS in living cells. ONOO^– can activate luminescence of QDs in the absence of excitation source, effectively avoiding background noise and scattering of light from biological matrixes produced by in situ excitation; however, there is no response to other ROS including ¹O₂, H₂O₂, ^•OH, O₂^•–, and ClO^–. The outstanding selectivity of the present CL probe leads us to detect the exogenous release of ONOO^– from 3-morpholinosydnonimine (SIN–1) in living cells. These results suggest that this present probe-based CL provides a promising platform for highly selective and sensitive detection of ONOO^– in biological systems.