A Fluorescent Probe for Ratiometric Imaging of SO2 Derivatives in Mitochondria of Living Cells

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• 作者：Haidong Li ; Qichao Yao ; Jiangli Fan ; Chong Hu ; Feng Xu ; Jianjun Du ; Jingyun Wang ; Xiaojun Peng
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2016
• 出版时间：February 17, 2016
• 年：2016
• 卷：55
• 期：6
• 页码：1477-1483
• 全文大小：449K
• ISSN：1520-5045

文摘

When SO₂ is inhaled through the respiratory tract into the body, it can be easily hydrated to sulfite (SO₃^2–) and bisulfate (HSO₃^–) ions. They are widely used as essential preservatives for foods, beverages, and pharmaceutical products. Exposure to high doses of bisulfate induces a large number of respiratory diseases relevant to lung cancer, cardiovascular diseases, and many neurological disorders, as they involve in various physiology and pathological processes in mitochondria cell apparatus. In this work, a new fluorescent probe CY-SO₂ for bisulfate/sulfite based on hemicyanine dye is reported, which can used in solution detection with 56-fold fluorescence ratio (F_467 nm/F_580 nm) enhancement, fast response (completed within 90 s) and excellent sensitivity (DL 2.67 nM). The effect have been exhibited in real sugar samples tests. CY-SO₂ also displayed fluorescent imaging of breast cancer cells (MCF-7) successfully, demonstrating that the probe is a novel mitochondria-targeted ratiometric fluorescent probe to image cellular SO₂ derivatives.