New Analytical Tool for the Detection of Ractopamine Abuse in Goat Skeletal Muscle by Potential Gene Expression Biomarkers

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• 作者：Luyao Zhao ; Shuming Yang ; Yanhua Zhang ; Ying Zhang ; Can Hou ; Yongyou Cheng ; Xinyong You ; Xu Gu ; Zhen Zhao ; Tunio Muhammad Tarique
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：March 2, 2016
• 年：2016
• 卷：64
• 期：8
• 页码：1861-1867
• 全文大小：380K
• ISSN：1520-5118

文摘

In this study, quantification of mRNA gene expression was examined as biomarkers to detect ractopamaine abuse and ractopamaine residues in cashmere goats. It was focused on the identification of potential gene expression biomarkers and describing the coreletionship between gene expression and residue level by 58 animals for 49 days. The results showed that administration periods and residue levels significantly influenced mRNA expressions of the β₂-adrenergic receptor (β₂AR), the enzymes PRKACB, ADCY3, ATP1A3, ATP2A3, PTH, and MYLK, and the immune factors IL-1β and TNF-α. Statistical analysis like principal components analysis (PCA), hierarchical cluster analysis (HCA), and discriminant analysis (DA) showed that these genes can serve as potential biomarkers for ractopamine in skeletal muscle and that they are also suitable for describing different residue levels separately.