Anti-inflammatory Cerebrosides from Cultivated Cordyceps militaris

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• 作者：Ching-Peng Chiu ; Shan-Chi Liu ; Chih-Hsin Tang ; You Chan ; Mohamed El-Shazly ; Chia-Lin Lee ; Ying-Chi Du ; Tung-Ying Wu ; Fang-Rong Chang ; Yang-Chang Wu
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：February 24, 2016
• 年：2016
• 卷：64
• 期：7
• 页码：1540-1548
• 全文大小：468K
• ISSN：1520-5118

文摘

Cordyceps militaris (bei-chong-chaw, northern worm grass) is a precious and edible entomopathogenic fungus, which is widely used in traditional Chinese medicine (TCM) as a general booster for the nervous system, metabolism, and immunity. Saccharides, nucleosides, mannitol, and sterols were isolated from this fungus. The biological activity of C. militaris was attributed to the saccharide and nucleoside contents. In this study, the aqueous methanolic fraction of C. militaris fruiting bodies exhibited a significant anti-inflammatory activity. Bioactivity-guided fractionation of the active fraction led to the isolation of eight compounds, including one new and two known cerebrosides (ceramide derivatives), two nucleosides, and three sterols. Cordycerebroside A (1), the new cerebroside, along with soyacerebroside I (2) and glucocerebroside (3) inhibited the accumulation of pro-inflammatory iNOS protein and reduced the expression of COX-2 protein in LPS-stimulated RAW264.7 macrophages. This is the first study on the isolation of cerebrosides with anti-inflammatory activity from this TCM.