Loading of Antibiotics into Polyelectrolyte Multilayers after Self-Assembly and Tunable Release by Catechol Reaction

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• 作者：Bailiang Wang ; Zi Ye ; Yihong Tang ; Huihua Liu ; Quankui Lin ; Hao Chen ; Kaihui Nan
• 刊名：Journal of Physical Chemistry C
• 出版年：2016
• 出版时间：March 24, 2016
• 年：2016
• 卷：120
• 期：11
• 页码：6145-6155
• 全文大小：662K
• ISSN：1932-7455

文摘

The colonization of bacteria on biomedical implants and subsequent forming of biofilm often leads to serious infections. In this work, poly(acrylic acid) modified by dopamine (PAA-dopa) and poly(ethylenimine) (PEI) self-assembled into multilayer films were used to load and for sustained release of antibiotics. The obtained multilayer films were immersed in tris-buffered saline containing gentamicin sulfate (GS) to load the antibiotics and were cross-linked via catechol chemistry of dopa. Through raising pH value, more carboxyl groups of PAA were protonated and combined with GS through electrostatic forces. After cross-linking, the multilayer films were used as a drug delivery system to embed GS in the matrices which showed a two-stage sustained release profile. In vitro, the release of GS into phosphate-buffered saline (PBS) from the multilayer films, as well as the antibacterial activity, was determined. The drug-loaded multilayer films showed excellent bactericidal function against Staphylococcus aureus (S. aureus) and good cell compatibility toward human lens epithelial cells when the loading dosage of GS was 255 μg/cm². In vivo, multilayer films of modified and uncoated polydimethylsiloxane (PDMS) were implanted into subcutaneous incisions of New Zealand white rabbits. Both wound healing appearance evaluation and histopathology analysis demonstrated that implantation of the antibacterial coating modified PDMS had promoted wound healing and showed anti-inflammatory effect. In summary, this study presents a convenient and effective method for the incorporation of antibacterial agents into multilayer films.