Chemisorption Mechanism of DNA on Mg/Fe Layered Double Hydroxide Nanoparticles: Insights into Engineering Effective SiRNA Delivery Systems

详细信息    查看全文

• 作者：Mingsheng Lu ; Zhi Shan ; Kori Andrea ; Bruce MacDonald ; Stefanie Beale ; Dennis E. Curry ; Li Wang ; Shujun Wang ; Ken D. Oakes ; Craig Bennett ; Wenhui Wu ; Xu Zhang
• 刊名：Langmuir
• 出版年：2016
• 出版时间：March 22, 2016
• 年：2016
• 卷：32
• 期：11
• 页码：2659-2667
• 全文大小：550K
• ISSN：1520-5827

文摘

Layered double hydroxide nanoparticles (LDH NPs) have attracted interest as an effective gene delivery vehicle in biomedicine. Recent advances in clinic trials have demonstrated the efficacy of Mg/Fe LDHs for hyperphosphatemia treatment, but their feasibility for gene delivery has not been systematically evaluated. As a starting point, we aimed to study the interaction between oligo-DNA and Mg/Fe LDH NPs. Our investigation revealed the chemisorption mechanism of DNA on Mg/Fe LDH surfaces, wherein the phosphate backbone of the DNA polymer coordinates with the metal cations of the LDH lattice via the ligand-exchange process. This mechanistic insight may facilitate future gene delivery applications using Mg/Fe LDH NPs.