Enhanced Osteogenic Activity of TiO2 Nanorod Films with Microscaled Distribution of Zn-CaP

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• 作者：Meng He ; Xiaoyi Chen ; Kui Cheng ; Wenjian Weng ; Huiming Wang
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：March 23, 2016
• 年：2016
• 卷：8
• 期：11
• 页码：6944-6952
• 全文大小：596K
• ISSN：1944-8252

文摘

The topography at the micro-/nanoscale level and bioactive composition of material surfaces have been thought to play vital roles in their interactions with cells. However, it is still a challenge to further modify special topography with biodegradable composition or vice versa. In this study, TiO₂ nanorod films covered with microscale-distributed Zn-containing calcium phosphate (Zn-CaP) were prepared, trying to create a micro-/nanoscale topography and Zn²⁺ release capability. MC3T3-E1 cells cultured on TiO₂ nanorod film with sparsely distributed Zn-CaP (TiO₂/S-ZCP) had significantly higher biological responses than those on the films with densely distributed Zn-CaP (TiO₂/D-ZCP) and fully covered Zn-CaP (F-ZCP). TiO₂/S-ZCP film was demonstrated to facilitate osteogenic differentiation much more strongly than F-ZCP and TiO₂/D-ZCP films based on evaluations of ALP, related gene expressions, and extracellular matrix mineralization. The higher osteogenic differentiation on TiO₂/S-ZCP film is ascribed to the micro-/nanoscale topography from Zn-CaP coverage promoting cell adhesion and filopodia extension, and inducing differentiation-orientation in the initial stage. And consequently Zn²⁺ release results in enhancement of differentiation. Therefore, we believe that better organization of the micro-/nanotopography and bioactive ion release on the surface would be a promising way to enhance osteogenic activity for orthopedic and dental implants.