Thermoresponsive Epitope Surface-Imprinted Nanoparticles for Specific Capture and Release of Target Protein from Human Plasma

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• 作者：Senwu Li ; Kaiguang Yang ; Nan Deng ; Yi Min ; Lukuan Liu ; Lihua Zhang ; Yukui Zhang
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：March 9, 2016
• 年：2016
• 卷：8
• 期：9
• 页码：5747-5751
• 全文大小：320K
• ISSN：1944-8252

文摘

Among various artificial antibodies, epitope imprinted polymer has been paid increasingly attention. To modulate the “adsorption and release” behavior by environment stimuli, N-isopropylacrylamide, was adopted to fabricate the thermoresponsive epitope imprinted sites. The prepared imprinted materials could adsorb 46.6 mg/g of target protein with the imprinting factor of 4.0. The template utilization efficiency could reach as high as 8.21%. More importantly, in the real sample, the materials could controllably capture the target protein from the human plasma at 45 °C and release it at 4 °C, which demonstrated the “on-demand” application potentials of such materials in the biomolecule recognition field.