Lactose-Functionalized Gold Nanorods for Sensitive and Rapid Serological Diagnosis of Cancer

详细信息    查看全文

• 作者：Yuetao Zhao ; Liping Tong ; Yong Li ; Haobo Pan ; Wei Zhang ; Min Guan ; Weihao Li ; Yixin Chen ; Qing Li ; Zhongjun Li ; Huaiyu Wang ; Xue-Feng Yu ; Paul K. Chu
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：March 9, 2016
• 年：2016
• 卷：8
• 期：9
• 页码：5813-5820
• 全文大小：575K
• ISSN：1944-8252

文摘

Timely and accurate diagnosis of cancer is crucial to cancer treatment. However, serological diagnosis of cancer still faces great challenge because the conventional methodology based on the enzyme-linked immune sorbent assay (ELISA) is costly, time-consuming, and complicated, involving multiple steps. Herein, lactose-functionalized gold nanorods (Lac-GNRs) are fabricated as efficient biosensors to detect cancerous conditions based on the unique surface plasmon resonance properties of GNRs and high specificity of lactose to the galectin-1 cancer biomarker. A trace concentration of galectin-1 as small as 10^–13 M can be detected by Lac-GNRs. The comparative study among BSA, galectin-3, and galectin-1 demonstrates the good specificity of Lac-GNRs to galectin-1 either in aqueous solutions or in the complex and heterogeneous serum specimens. Clinical tests show that the Lac-GNRs biosensors can readily distinguish the serums of cancer patients from those of healthy persons simply by using a microplate reader or even direct visual observation. The Lac-GNRs biosensing platform is highly efficient and easy to use and have great potential in rapid screening of cancer patients.