Multiple-Armed Tetrahedral DNA Nanostructures for Tumor-Targeting, Dual-Modality in Vivo Imaging

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• 作者：Dawei Jiang ; Yanhong Sun ; Jiang Li ; Qian Li ; Min Lv ; Bing Zhu ; Tian Tian ; Dengfeng Cheng ; Jiaoyun Xia ; Lan Zhang ; Lihua Wang ; Qing Huang ; Jiye Shi ; Chunhai Fan
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：February 24, 2016
• 年：2016
• 卷：8
• 期：7
• 页码：4378-4384
• 全文大小：457K
• ISSN：1944-8252

文摘

In this work, we have developed multiple-armed DNA tetrahedral nanostructures (TDNs) for dual-modality in vivo imaging using near-infrared (NIR) fluorescence and single-photon emission computed tomography (SPECT). We found that the presence of arm strands in TDNs remarkably enhanced their in vitro stability, allowing them to stay intact for at least 12 h in serum. By using NIR fluorescence imaging, we evaluated in mice the pharmacokinetics of TDNs, which exhibited distinctly different in vivo biodistribution patterns compared with those of double-stranded (ds)DNA. We also noticed that TDNs had twofold longer circulation time in the blood system than that of dsDNA. With the use of multiple-armed TDNs, we could precisely anchor an exact number of functional groups including tumor-targeting folic acid (FA), NIR emitter Dylight 755, and radioactive isotope ^99mTc on prescribed positions of TDNs, which showed the capability of targeted imaging ability in cancer cells. Furthermore, we realized noninvasive tumor-targeting imaging in tumor-bearing mice by using both NIR and SPECT modalities.