Postsynthetic Modification of DNA Phosphodiester Backbone for Photocaged DNAzyme

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• 作者：Xiaoyan Wang ; Mengli Feng ; Lu Xiao ; Aijun Tong ; Yu Xiang
• 刊名：ACS Chemical Biology
• 出版年：2016
• 出版时间：February 19, 2016
• 年：2016
• 卷：11
• 期：2
• 页码：444-451
• 全文大小：502K
• ISSN：1554-8937

文摘

Photocaged (photoactivatable) biomolecules are powerful tools for noninvasive control of biochemical activities by light irradiation. DNAzymes (deoxyribozymes) are single-stranded oligonucleotides with a broad range of enzymatic activities. In this work, to construct photocaged DNAzymes, we developed a facile and mild postsynthetic method to incorporate an interesting photolabile modification (thioether-enol phosphate, phenol substituted, TEEP–OH) into readily available phosphorothioate DNA. Upon light irradiation, TEEP–OH transformed into a native DNA phosphodiester, and accordingly the DNAzymes with RNA-cleaving activities were turned “on” from its inactive and caged form. Activation of the TEEP–OH-caged DNAzyme by light was also successful inside live cells.