Fluorogenic Enhancement of an in Vitro-Selected Peptide Ligand by Replacement of a Fluorescent Group

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• 作者：Wei Wang ; Liping Zhu ; Yoshinori Hirano ; Marziyeh Kariminavargani ; Seiichi Tada ; Guanxin Zhang ; Takanori Uzawa ; Deqing Zhang ; Takuji Hirose ; Makoto Taiji ; Yoshihiro Ito
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：August 16, 2016
• 年：2016
• 卷：88
• 期：16
• 页码：7991-7997
• 全文大小：396K
• 年卷期：0
• ISSN：1520-6882

文摘

To prepare a fluorogenic peptide ligand which binds to an arbitrary target, we previously succeeded in seeking a fluorogenic ligand to calmodulin using in vitro selection. In this study the environment-sensitive fluorescent group in the selected peptide ligand was replaced with other fluorescent groups to find the possibility to increase the fluorogenic activity. Surface plasmon resonance measurement exhibited that the binding affinity was held even after the replacement. However, the replacement significantly affected the fluorogenic activity. It depended on the kind of incorporated fluorophors and linker length. As a result, the incorporation of 4-N,N-dimethylamino-1,8-naphthalimide enhanced the fluorescence intensity over 100-fold in the presence of target calcium-bound calmodulin. This study demonstrated that the functionality of in vitro selected peptide can be tuned with keeping the binding affinity.